By mass spectrometry
Identity
Mass spec measures the molecular weight of what is in the sample. If it matches the expected weight of the sequence, you have confirmation that the right peptide was made.
TIER 1 SCIENCE
Know What’s in the Vial Before It Reaches Your Bench.
A plain guide to how research peptides are made, tested and documented, and what a Certificate of Analysis should tell you before you order.
RUOAll products referenced are for in vitro laboratory research only. Not for human or veterinary use.
THE BASICS
Short Chains. Precise Sequences.
A peptide is a short chain of amino acids linked in a specific order. Change one amino acid in that sequence and you have a different molecule. That is why identity and purity matter so much in a lab setting. If the material on your bench is not the sequence on the label, the data that comes out of your experiment is not worth much.
Research peptides are made synthetically, usually one amino acid at a time on a solid support. The finished peptide is purified, then freeze-dried into a stable powder (lyophilized) so it holds up in storage and transit.
01
Solid support
Chain is anchored to a resin bead
02
One at a time
Amino acids added in sequence
03
Purified
Finished peptide purified
04
Lyophilized
Freeze-dried to a stable powder
VERIFICATION
Two Questions Every Batch Should Answer.
Good testing answers two separate questions. Is this the right molecule, and how much of the sample is that molecule? Different instruments answer each one.
By HPLC
Purity
High-performance liquid chromatography separates the target peptide from anything else in the sample, such as incomplete or truncated chains. The result is a percentage. Higher is cleaner.
Third-party testing
Independent review
Testing done by an outside lab (Freedom Diagnostics), not the seller, removes the obvious conflict of interest.
READ THE PAPERWORK
A COA Is Only Useful If You Know Where to Look.
A Certificate of Analysis is the lab’s report on a specific batch. Before you trust one, check that it includes these six things.
01
Batch or lot number
It should match the number on the vial label. A COA with no lot number could belong to any batch.
02
Test date
Recent and specific. A report from years ago tells you little about today’s stock.
03
Testing lab
The name of the lab that ran the analysis, ideally independent from the seller.
04
Identity result
The measured molecular weight next to the expected value.
05
Purity result
An HPLC percentage, with the chromatogram attached if possible.
06
Appearance and quantity
What the sample looked like and how much was tested.
If a seller won’t show you the COA before you buy, treat that as your answer.
HANDLING
Good Material Can Still Be Ruined on the Shelf.
Lyophilized peptides are stable, but heat, light and moisture break them down over time. Most labs store them cold, sealed and away from light, with long-term stock kept frozen. When a vial comes out of cold storage, let it reach room temperature before opening so condensation doesn’t form inside. Label everything with the lot number and date received so it can be matched to its COA later.
Cold, sealed, away from light
Long-term stock kept frozen
Room temperature before opening
So condensation doesn’t form inside
Lot number and date received
So it can be matched to its COA later
Tier 1 vial storage cases keep vials organized and protected between uses. Shop storage cases ↗
WHERE TIER 1 COMES IN
We Hold Our Own Inventory to This Checklist.
Everything on this page is how we think research material should be sourced. It is also how Tier 1 Peptides runs.
Third-party tested
Every product is tested by an independent lab (Freedom Diagnostics) for identity and purity.
Lab Reports Before You Buy
Lab reports are published on the site, not handed over only on request.
Consistent batch to batch
Quality control checks keep each new batch to the same standard as the last, maintaining a minimum purity of 99% or higher.
Research grade only
Sold for in vitro laboratory research. Not for human or veterinary use.
Fast U.S. shipping
Orders ship from New York with free shipping over $200.
FAQ
Frequently Asked Questions
What does “research use only” mean?
It means the product is sold for in vitro laboratory research, such as cell culture or analytical work. It is not a drug, supplement or cosmetic, and it is not for use in humans or animals.
What’s the difference between purity and identity?
Identity confirms the right molecule was made. Purity tells you what percentage of the sample is that molecule. You need both. A sample can be the right peptide at low purity, or high purity of the wrong one.
What purity should I look for?
It depends on the work, but many labs look for 98% or higher by HPLC. Whatever the number, it should come from a named lab and match the batch you receive.
Why does lyophilized form matter?
Freeze-drying removes water, which is what makes peptides break down fastest. Powder holds up far better in storage and shipping than a solution does.
Can I see test results before ordering from Tier 1?
Yes. Lab reports are published on the Tier 1 Peptides site. View lab results ↗
Do you give guidance on how to use these products?
No. We provide documentation on identity, purity and storage only. Experimental design is the responsibility of the researcher and their institution.
Where do you ship?
Tier 1 ships within the United States only.
What is your return policy?
To protect the integrity of every product, all sales are final.
Check the Paperwork. Then Decide.
You know what a good COA looks like now. See how ours measure up.